Probiotics Microsphere Restored Gut Barrier by Reducing Oxidative Stress in Colitis

Author(s):

Cheng Zhao; Jianan Ren

Background:

The gut microflora dysbiosis and the damage to gut barrier have been closely related with the colitis. Probiotics could interact with the intestinal epithelium cells and dendritic cells modulating inflammation. In this study, the effect of encapsuled probiotics on the gut barrier was evaluated by DSS induced colitis model.

Hypothesis:

The encapsuled probiotics could ameliorate the gut barrier through reducing the inflammation and oxidative stress.

Methods:

The microsphere was produced and characterized. The probiotics was encapsuled. The rat model was induced by DSS. The expression of gut barrier protein was analyzed by WB, qPCR and fluorescent staining. HE staining was also conducted. Oxidative sterss was measured through the level of MDA, SOD and GSH-Px. TUNNEL assay was conducted to make out the apoptosis.

Results:

The result supported that the encapsuled probiotics could reduce the level of oxidative stress and inflammation. It could modulate the expression of inflammatory cytokines. At last, it increased the level of gut barrier protein which prevents furthur damage in return.

Conclusions:

The probiotics microsphere could modulate the oxidative stress and inflammatory level. It could avoid the damage to gut barrier and prevent furthur damage. The probiotics microsphere could be used as a encouraging method to protect the gut barrier against inflammation.